Part:BBa_K4806203:Design

CYP3A4 gene for expression in the chloroplast for Chlamydomonas reinhardtii (Phytobrick)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 2028
  Illegal PstI site found at 2350
  Illegal PstI site found at 2410
  Illegal PstI site found at 2882
  Illegal PstI site found at 2951
  Illegal PstI site found at 3055
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 2028
  Illegal PstI site found at 2350
  Illegal PstI site found at 2410
  Illegal PstI site found at 2882
  Illegal PstI site found at 2951
  Illegal PstI site found at 3055
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 2028
  Illegal PstI site found at 2350
  Illegal PstI site found at 2410
  Illegal PstI site found at 2882
  Illegal PstI site found at 2951
  Illegal PstI site found at 3055
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 2028
  Illegal PstI site found at 2350
  Illegal PstI site found at 2410
  Illegal PstI site found at 2882
  Illegal PstI site found at 2951
  Illegal PstI site found at 3055
  Illegal NgoMIV site found at 2272
  Illegal NgoMIV site found at 3774
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

• 1. PSAD-promotor (A1-B1) (BBa_K4806010)
• 2. CTPPSAD-transit peptide (B2) (BBa_K4806014)
• 3. CYP3A4 gene (B3-B4) (BBa_K4806000)
• 4. HA-tag (B5) (BBa_K3002017)^*
• 5. tRPL23-terminator (B6-C1) (BBa_K3002006)^*

Additionally, connectors were used to create the MoClo overhangs:

• 1. Connector B1-B2 (BBa_K3002302)^*
• 2. Connector B2-B3 (BBa_K3002303)^*
• 3. Connector B4-B5 (BBa_K3002304)^*
• 4. Connector B5-B6 (BBa_K3002305)^*

The ^* marked parts were not created by us. Our results can be found on the experience page of each part.